Tracking Hepatitis B Virus - Like Vesicles in Living Cells
نویسنده
چکیده
We tracked the Hepatitis B virus-like vesicles (the surface antigen of Hepatitis B virus, HBsAg) infecting living cells by real-time laser confocal fluorescence imaging. The results show a vesicle-associated movement in the early stage of endocytic trafficking of HBsAg near the plasma membrane. Tracking the internalized HBsAg in the later stage of infection reveals that most HBsAg particles are colocalized with the carrier vesicles and show a related motion in living cells. The endocytosis of HBsAg and formation of carrier vesicles are both dependent on actin filaments. Our findings may offer new insights into the different stages of HBsAg infection and the functions of carrier vesicles in virus-related transport, and may provide an approach that can be used for a variety of enveloped
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